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Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study

Received: 23 May 2021     Accepted: 5 July 2021     Published: 15 July 2021
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Abstract

Background and Aim: Oral squamous cell carcinoma (OSCC) is a growing health problem worldwide, for which standard treatment strategies have failed to increase its recovery rate significantly. In recent decades, many studies have investigated herbal or natural compounds for the prevention and treatment of OSCC. The present study was performed to investigate the effects of the extract of Portulaca oleracea seed, Berberis vulgaris, and Vaccinium myrtillus fruit on the OSCC. Experimental procedure: Firstly, the herbal samples were purchased from the medicinal plant market and each one of the samples was extracted. Afterward, SCC-15 cells were purchased from the Iranian Biological Resource Center. MTT test was used to evaluate cell proliferation and to measure the transcription rate of ki67, miR-4492, and miR-877-5p genes using Real-time PCR. Finally, the western blot test was used to measure the translation rate of b-cell lymphoma 2 and matrix metalloproteinase-2 genes. Quantitative variables of the present study were described as mean and standard deviation and the three-way ANOVA was used via SPSS ver. 25. an independent t-test was used. P-value< 0.05 was considered a statistically significant level. Results and Conclusion: Briefly, the findings of the present study showed that all three extracts had significant effects on growth suppression, apoptosis induction, and OSCC metastasis. Also, the triple (PVB) combination and Portulaca-Berberis (PB) combination were shown to be the most effective. This result indicates the anti-cancer synergistic effect of these extracts especially P and B, on suppressing OSCC, which should be confirmed in future animal and human studies.

Published in Rehabilitation Science (Volume 6, Issue 3)
DOI 10.11648/j.rs.20210603.11
Page(s) 41-48
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Herbal Compound, Apoptosis, Cell Proliferation, Neoplasm Metastasis, Micro RNA

References
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    Sara Pourshahidi, Mohaddeseh Davari, Naghmeh Bahrami, Roja Rahimi. (2021). Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study. Rehabilitation Science, 6(3), 41-48. https://doi.org/10.11648/j.rs.20210603.11

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    ACS Style

    Sara Pourshahidi; Mohaddeseh Davari; Naghmeh Bahrami; Roja Rahimi. Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study. Rehabil. Sci. 2021, 6(3), 41-48. doi: 10.11648/j.rs.20210603.11

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    AMA Style

    Sara Pourshahidi, Mohaddeseh Davari, Naghmeh Bahrami, Roja Rahimi. Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study. Rehabil Sci. 2021;6(3):41-48. doi: 10.11648/j.rs.20210603.11

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  • @article{10.11648/j.rs.20210603.11,
      author = {Sara Pourshahidi and Mohaddeseh Davari and Naghmeh Bahrami and Roja Rahimi},
      title = {Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study},
      journal = {Rehabilitation Science},
      volume = {6},
      number = {3},
      pages = {41-48},
      doi = {10.11648/j.rs.20210603.11},
      url = {https://doi.org/10.11648/j.rs.20210603.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.rs.20210603.11},
      abstract = {Background and Aim: Oral squamous cell carcinoma (OSCC) is a growing health problem worldwide, for which standard treatment strategies have failed to increase its recovery rate significantly. In recent decades, many studies have investigated herbal or natural compounds for the prevention and treatment of OSCC. The present study was performed to investigate the effects of the extract of Portulaca oleracea seed, Berberis vulgaris, and Vaccinium myrtillus fruit on the OSCC. Experimental procedure: Firstly, the herbal samples were purchased from the medicinal plant market and each one of the samples was extracted. Afterward, SCC-15 cells were purchased from the Iranian Biological Resource Center. MTT test was used to evaluate cell proliferation and to measure the transcription rate of ki67, miR-4492, and miR-877-5p genes using Real-time PCR. Finally, the western blot test was used to measure the translation rate of b-cell lymphoma 2 and matrix metalloproteinase-2 genes. Quantitative variables of the present study were described as mean and standard deviation and the three-way ANOVA was used via SPSS ver. 25. an independent t-test was used. P-value< 0.05 was considered a statistically significant level. Results and Conclusion: Briefly, the findings of the present study showed that all three extracts had significant effects on growth suppression, apoptosis induction, and OSCC metastasis. Also, the triple (PVB) combination and Portulaca-Berberis (PB) combination were shown to be the most effective. This result indicates the anti-cancer synergistic effect of these extracts especially P and B, on suppressing OSCC, which should be confirmed in future animal and human studies.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study
    AU  - Sara Pourshahidi
    AU  - Mohaddeseh Davari
    AU  - Naghmeh Bahrami
    AU  - Roja Rahimi
    Y1  - 2021/07/15
    PY  - 2021
    N1  - https://doi.org/10.11648/j.rs.20210603.11
    DO  - 10.11648/j.rs.20210603.11
    T2  - Rehabilitation Science
    JF  - Rehabilitation Science
    JO  - Rehabilitation Science
    SP  - 41
    EP  - 48
    PB  - Science Publishing Group
    SN  - 2637-594X
    UR  - https://doi.org/10.11648/j.rs.20210603.11
    AB  - Background and Aim: Oral squamous cell carcinoma (OSCC) is a growing health problem worldwide, for which standard treatment strategies have failed to increase its recovery rate significantly. In recent decades, many studies have investigated herbal or natural compounds for the prevention and treatment of OSCC. The present study was performed to investigate the effects of the extract of Portulaca oleracea seed, Berberis vulgaris, and Vaccinium myrtillus fruit on the OSCC. Experimental procedure: Firstly, the herbal samples were purchased from the medicinal plant market and each one of the samples was extracted. Afterward, SCC-15 cells were purchased from the Iranian Biological Resource Center. MTT test was used to evaluate cell proliferation and to measure the transcription rate of ki67, miR-4492, and miR-877-5p genes using Real-time PCR. Finally, the western blot test was used to measure the translation rate of b-cell lymphoma 2 and matrix metalloproteinase-2 genes. Quantitative variables of the present study were described as mean and standard deviation and the three-way ANOVA was used via SPSS ver. 25. an independent t-test was used. P-value< 0.05 was considered a statistically significant level. Results and Conclusion: Briefly, the findings of the present study showed that all three extracts had significant effects on growth suppression, apoptosis induction, and OSCC metastasis. Also, the triple (PVB) combination and Portulaca-Berberis (PB) combination were shown to be the most effective. This result indicates the anti-cancer synergistic effect of these extracts especially P and B, on suppressing OSCC, which should be confirmed in future animal and human studies.
    VL  - 6
    IS  - 3
    ER  - 

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Author Information
  • Department of Oral & Maxillofacial Medicine, Tehran University of Medical Sciences, Tehran, Iran

  • Department of Oral & Maxillofacial Medicine, Semnan University of Medical Sciences, Semnan, Iran

  • Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran

  • Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran

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